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Philips EM400T — Transmission electron microscope (TEM)
Research Center of Electron Microscopy

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In recent years Transmission Electron Microscopy (TEM) has proved to be one of the most powerful techniques to directly elucidate the structural organisation of supramolecular assemblies on the nanometer scale and beyond. A quantum leap in the field was the development of cryo-techniques, in particular cryo-TEM, which allows to study ultrastructures of self-assembled organic materials under native conditions e.g in the presence of the solvent in which the self-assembly takes place. This development is of special importance as the ultra structural organisation of molecules emerging in a supramolecular aggregation process differs in most cases significantly from its organisation in the crystal compound. Therefore, X-ray diffraction based techniques, which are indispensable in the field of protein structure analysis, are of less or even no use in the field of supramolecular chemistry.
Moreover, the recent developments in instrumentation and computation, in particular the tomographical and image processing techniques, have further sustained the importance of TEM as they help to make detailed three-dimensional structural information available.    » more



 

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Last updated: 15.07.2011